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Old 03-29-2009   #1 (permalink)
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Using TLC for measuring quantities

I am studying plants a fair bit now, and a current project is comparing certain plants' chemicals and quantities. I am fairly able to work with tlc plates and reagents, and identifying some of the chemicals (still working on this, but a lot has to do with just playing and comparing with others work and reference samples etc).

But one thing i am still struggling to comprehend is the analysis in getting the quantity using TLC . i know its not as accurate as other methods....but i have no lab so cost of columns and better equipment is a big limiting factor!

Anyone here have experience with this?


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Stephen Robert Irwin: 22 February 1962 – 4 September 2006. Rest In Peace.

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