I've never had any experience with quantifying compounds using TLC since I've always had access to more complex equipment.
The only ways I can think of that would allow you to measure quantity from your TLC plate would be to:
- Scrape off the spot, dissolve in solvent and measure by some other technique (like spectroscopy)
- Use scanning densitometer
Unfortunately, these methods require access to a lab. I really can't think of any way for you to get an idea without more advanced techniques. Sorry.

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