New Plants/Micropropagation/Somatic Cell Fusion

[vanamoinen1]

Does anyone here have experience with somatic cell fusion (plants) and the generation of adult plants therefrom? Specifically, what guidelines/texts/papers can you recommend for determining suitability of the technique given particular plants, experimental parameters including equipment, protocol and task-planning, and end-products--i.e. how can I learn of successful uses of this technique?

I'd also be interested in hearing from members who have experience in anther culture and the development of haploids therefrom. For that matter, I'm always interested in learning of your micropropagation experiences.

Finally, I'd also be interested to learn of any artificial stigmatic fluid preparations which might be used in situations where a desirable plant cultivar (which otherwise would be useful in planning plant crosses) is incapable of producing its own fluid. I do know that boron is essential in both the anther culture issue and this one, as it is necessary for the growth of pollen tubules, but otherwise, I have no references to guide me to develop an artificial stigmatic fluid for Hemerocallis (daylily).

I know it's a tall order, so thanks in advance!