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Old 01-13-2008   #1 (permalink)
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Reverse phase HPLC and end-capping

Am still revising but have confused myself somewhat over whether the above are the same thing or two different things.

In my notes I have:
normal phase silica gel with Si-O-SiOC18H37 groups and SiOH active sites for interaction with analytes and called that ODS1.

reverse phase HPLC as being chemically modified and having Si-OSiOCH3 groups on the active sites and called that ODS2.

I then have notes describing end-capping as the reaction of organochlorosilanes with silanol groups and an equation of SiOH + C18H37SiCl3 being silylated and hydrolysed to Si-OSiC18H37 (+ 2HCl) but thats the same as the groups on the NPHPLC.

If the active sites on RP HPLC are already modified how can this end-capping occur? Am confusing myself even more and can't even get the right words for my questions to make sense! Can anyone help again! Thank you.

Edit: Found the answer but can't delete this post - sorry! Will leave it in case anyone wants to answer anyway!


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Last edited by joho; 01-13-2008 at 07:56 AM. Reason: Found the answer!
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