04-12-2007
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 Curious |  Dissociation constant Can anyone help me?  I am trying to purify a protein out of a nuclear extract. I know absolutely nothing about this protein (transcription factor), except which DNA sequence it binds to. I try to follow the purification through a gel shift (EMSA) assay and have heard that you can calculate the dissociation constant (Kd) from gel shifts. Do anyone know how  I would be thankful for all your help! /Anna Imagination is more important than knowledge... Albert Einstein | |
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